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Opinion: hazards faced by macromolecules when confined to thin aqueous films

Samples prepared for single-particle electron cryo-microscopy (cryo-EM) necessarily have a very high surface-to-volume ratio during the short period of time between thinning and vitrification. During this time, there is an obvious risk that macromolecules of interest may adsorb to the air–water inte...

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Bibliographic Details
Published in:Biophys Rep
Main Authors: Glaeser, Robert M., Han, Bong-Gyoon
Format: Artigo
Language:Inglês
Published: Springer Berlin Heidelberg 2016
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5516009/
https://ncbi.nlm.nih.gov/pubmed/28781996
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s41048-016-0026-3
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