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Streptavidin crystals as nanostructured supports and image-calibration references for cryo-EM data collection
For cryo-EM structural studies we seek to image membrane proteins as single particles embedded in proteoliposomes. One technical difficulty has been the low density of liposomes that can be trapped in the ~100 nm ice layer that spans holes in the perforated carbon support film of EM grids. Inspired...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3547683/ https://ncbi.nlm.nih.gov/pubmed/18707004 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2008.07.008 |
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