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Liposomes on a streptavidin crystal: a system to study membrane proteins by cryo-EM
In this chapter we describe the preparation of cryo-EM specimens for random spherically-constrained (RSC) single-particle reconstruction of membrane proteins. The specimen consists of liposomes into which the purified membrane protein is reconstituted at low density. The substrate is a 2D streptavid...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3903115/ https://ncbi.nlm.nih.gov/pubmed/20887857 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0076-6879(10)81007-9 |
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