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Automatic cryo-EM particle selection for membrane proteins in spherical liposomes

Random spherically constrained (RSC) single particle reconstruction is a method to obtain structures of membrane proteins embedded in lipid vesicles (liposomes). As in all single-particle cryo-EM methods, structure determination is greatly aided by reliable detection of protein “particles” in microg...

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Detalhes bibliográficos
Main Authors: Liu, Yunhui, Sigworth, Fred J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3978669/
https://ncbi.nlm.nih.gov/pubmed/24468290
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2014.01.004
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