Statistical Modeling and Removal of Lipid Membrane Projections for Cryo-EM Structure Determination of Reconstituted Membrane Proteins

This paper describes steps in the single-particle cryo-EM 3D structure determination of membrane proteins in their membrane environment. Using images of the Kv1.2 potassium-channel complex reconstituted into lipid vesicles, we describe procedures for the merging of focal-pairs of exposures and the r...

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Vydáno v:J Struct Biol
Hlavní autoři: Jensen, Katrine Hommelhoff, Brandt, Sami Sebastian, Shigematsu, Hideki, Sigworth, Fred J.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2016
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4866491/
https://ncbi.nlm.nih.gov/pubmed/26835990
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2016.01.012
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