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Streptavidin crystals as nanostructured supports and image-calibration references for cryo-EM data collection

For cryo-EM structural studies we seek to image membrane proteins as single particles embedded in proteoliposomes. One technical difficulty has been the low density of liposomes that can be trapped in the ~100 nm ice layer that spans holes in the perforated carbon support film of EM grids. Inspired...

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Dettagli Bibliografici
Autori principali: Wang, Liguo, Ounjai, Puey, Sigworth, Fred J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2008
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3547683/
https://ncbi.nlm.nih.gov/pubmed/18707004
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2008.07.008
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