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Creating a RAW264.7 CRISPR-Cas9 Genome Wide Library
The bacterial clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 genome editing tools are used in mammalian cells to knock-out specific genes of interest to elucidate gene function. The CRISPR-Cas9 system requires that the mammalian cell expresses Cas9 endonuclease, guide RNA (g...
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| Publicat a: | Bio Protoc |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Bio-Protocol
2017
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5580966/ https://ncbi.nlm.nih.gov/pubmed/28868328 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2320 |
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