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Rapid detection and isolation of covalent DNA/protein complexes: application to topoisomerase I and II.

A rapid and simple method has been developed which allows detection and isolation of covalent DNA/protein adducts. The method is based upon the use of an ionic detergent, SDS, to neutralize cationic sites of weakly bound proteins thereby resulting in their dissociation off the helix. Proteins tightl...

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Bibliografski detalji
Glavni autori: Trask, D K, DiDonato, J A, Muller, M T
Format: Artigo
Jezik:Inglês
Izdano: 1984
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC557405/
https://ncbi.nlm.nih.gov/pubmed/6325181
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