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Stabilization of type I topoisomerase-DNA covalent complexes by actinomycin D.

The activity of the endogenous DNA topoisomerase type I (EC 5.99.1.2) can be quantified in situ by determining how efficiently the enzyme is trapped in a covalent complex with DNA upon lysis of nuclei with detergents. In this way, we can measure relative levels of topoisomerase binding to DNA at nat...

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Detalhes bibliográficos
Main Authors: Trask, D K, Muller, M T
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC279782/
https://ncbi.nlm.nih.gov/pubmed/2830618
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