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Mutations in T7 RNA polymerase that support the proposal for a common polymerase active site structure.

In order to test the proposal that most nucleotide polymerases share a common active site structure and folding topology, we have generated 22 mutations of residues within motifs A, B and C of T7 RNA polymerase (RNAP). Characterization of these T7 RNAP mutants showed the following: (i) most of the m...

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Detalhes bibliográficos
Main Authors: Bonner, G, Patra, D, Lafer, E M, Sousa, R
Formato: Artigo
Idioma:Inglês
Publicado em: 1992
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC556837/
https://ncbi.nlm.nih.gov/pubmed/1396570
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