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Tunable recombinant protein expression in E. coli: promoter systems and genetic constraints

Tuning of transcription is a promising strategy to overcome challenges associated with a non-suitable expression rate like outgrowth of segregants, inclusion body formation, metabolic burden and inefficient translocation. By adjusting the expression rate—even on line—to purposeful levels higher prod...

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Detalhes bibliográficos
Publicado no:Appl Microbiol Biotechnol
Main Authors: Marschall, Lukas, Sagmeister, Patrick, Herwig, Christoph
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Berlin Heidelberg 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5566544/
https://ncbi.nlm.nih.gov/pubmed/27999902
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-016-8045-z
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