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Genetic selection system for improving recombinant membrane protein expression in E. coli

A major barrier to the physical characterization and structure determination of membrane proteins is low yield in recombinant expression. To address this problem, we have designed a selection strategy to isolate mutant strains of Escherichia coli that improve the expression of a targeted membrane pr...

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Bibliografske podrobnosti
Main Authors: Massey-Gendel, Elizabeth, Zhao, Anni, Boulting, Gabriella, Kim, Hye-Yeon, Balamotis, Michael A, Seligman, Len M, Nakamoto, Robert K, Bowie, James U
Format: Artigo
Jezik:Inglês
Izdano: Wiley Subscription Services, Inc., A Wiley Company 2009
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2708063/
https://ncbi.nlm.nih.gov/pubmed/19165721
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.39
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