Genetically encoded fluorescent sensors reveal dynamic regulation of NADPH metabolism

Reduced nicotinamide adenine dinucleotide phosphate (NADPH) is essential for for biosynthetic reactions and antioxidant functions; however, detection of NADPH metabolism in living cells remains technically challenging. We develop and characterize ratiometric, pH-resistant, genetically encoded fluore...

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Pubblicato in:Nat Methods
Autori principali: Tao, Rongkun, Zhao, Yuzheng, Chu, Huanyu, Wang, Aoxue, Zhu, Jiahuan, Chen, Xianjun, Zou, Yejun, Shi, Mei, Liu, Renmei, Su, Ni, Du, Jiulin, Zhou, Hai-Meng, Zhu, Linyong, Qian, Xuhong, Liu, Haiyan, Loscalzo, Joseph, Yang, Yi
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2017
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5555402/
https://ncbi.nlm.nih.gov/pubmed/28581494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4306
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