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The construction of siRNA plasmid targeting mouse HIF-1α and in vitro study of its inhibition effect

OBJECTIVE: To construct effective RNA-interference plasmids targeting mouse HIF-1α gene and testify their effects and specificities in interfering HIF-1α expression. METHODS: Three RNA-interference plasmids targeting mouse HIF-1α gene, pBS/U6/HIF-1α-siRNAI∼III, were constructed and identified using...

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Detalhes bibliográficos
Publicado no:Neurosci Bull
Main Authors: Ding, Zhen-Yu, Li, Ze-Gui, Xing, Yi-Zhan, Ji, Hua, Li, Hong-Li, Chang, Zhi-Jie
Formato: Artigo
Idioma:Inglês
Publicado em: Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5552566/
https://ncbi.nlm.nih.gov/pubmed/19448686
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12264-009-8122-8
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