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Elucidation of the Cryptic Methyl Group Epimerase Activity of Dehydratase Domains from Modular Polyketide Synthases Using a Tandem Modules Epimerase Assay

Dehydratase (DH) domains of cryptic function are often found in polyketide synthase (PKS) modules that produce epimerized (2S)-2-methyl-3-ketoacyl-ACP intermediates. A combination of tandem equilibrium isotope exchange (EIX) and a newly developed Tandem Modules Epimerase (TME) assay revealed the int...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:J Am Chem Soc
Prif Awduron: Xie, Xinqiang, Garg, Ashish, Khosla, Chaitan, Cane, David E.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2017
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5546871/
https://ncbi.nlm.nih.gov/pubmed/28682630
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.7b05502
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