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Elucidation of the Cryptic Epimerase Activity of Redox-Inactive Ketoreductase Domains from Modular Polyketide Synthases by Tandem Equilibrium Isotope Exchange

[Image: see text] Many modular polyketide synthases harbor one or more redox-inactive domains of unknown function that are highly homologous to ketoreductase (KR) domains. A newly developed tandem equilibrium isotope exchange (EIX) assay has now established that such “KR(0)” domains catalyze the bio...

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Autori principali: Garg, Ashish, Xie, Xinqiang, Keatinge-Clay, Adrian, Khosla, Chaitan, Cane, David E.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Chemical Society 2014
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4111212/
https://ncbi.nlm.nih.gov/pubmed/25004372
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja5056998
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