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An efficient method to enrich for knock-out and knock-in cellular clones using the CRISPR/Cas9 system

Clustered Regularly Interspaced Short Palindromic Repeats-associated protein 9 nuclease (CRISPR/Cas9) and Transcription Activator-Like Effector Nucleases (TALENs) are versatile tools for genome editing. Here we report a method to increase the frequency of Cas9-targeted cellular clones. Our method is...

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Foilsithe in:Cell Mol Life Sci
Main Authors: Niccheri, Francesca, Pecori, Riccardo, Conticello, Silvestro G.
Formáid: Artigo
Teanga:Inglês
Foilsithe: Springer International Publishing 2017
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5544813/
https://ncbi.nlm.nih.gov/pubmed/28421278
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00018-017-2524-y
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