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An ordered pathway of snRNP binding during mammalian pre-mRNA splicing complex assembly.
We have studied the assembly, composition and structure of splicing complexes using biotin-avidin affinity chromatography and RNase protection assays. We find that U1, U2, U4, U5 and U6 snRNPs associate with the pre-mRNA and are in the mature, functional complex. Association of U1 snRNP with the pre...
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| Publicado no: | EMBO J |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
1987
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC553648/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2959470/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1987.tb02520.x |
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