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Reconstituted mammalian U4/U6 snRNP complements splicing: a mutational analysis.

We have developed an in vitro complementation assay to analyse the functions of U6 small nuclear RNA (snRNA) in splicing and in the assembly of small nuclear ribonucleoproteins (snRNPs) and spliceosomes. U6-specific, biotinylated 2'-OMe RNA oligonucleotides were used to deplete nuclear extract...

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Detalhes bibliográficos
Publicado no:EMBO J
Main Authors: Wolff, T, Bindereif, A
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 1992
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC556455/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1740113/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1992.tb05057.x
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