Reconstituted mammalian U4/U6 snRNP complements splicing: a mutational analysis.

We have developed an in vitro complementation assay to analyse the functions of U6 small nuclear RNA (snRNA) in splicing and in the assembly of small nuclear ribonucleoproteins (snRNPs) and spliceosomes. U6-specific, biotinylated 2'-OMe RNA oligonucleotides were used to deplete nuclear extract of th...

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Pubblicato in:	EMBO J
Autori principali:	Wolff, T, Bindereif, A
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Nature Publishing Group 1992
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC556455/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1740113/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1992.tb05057.x
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