Quantification of cell cycle kinetics by EdU (5-ethynyl-2′-deoxyuridine)-coupled-fluorescence-intensity analysis

We propose a novel single-deoxynucleoside-based assay that is easy to perform and provides accurate values for the absolute length (in units of time) of each of the cell cycle stages (G1, S and G2/M). This flow-cytometric assay takes advantage of the excellent stoichiometric properties of azide-fluo...

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Detalhes bibliográficos
Publicado no:Oncotarget
Main Authors: Pereira, Pedro D., Serra-Caetano, Ana, Cabrita, Marisa, Bekman, Evguenia, Braga, José, Rino, José, Santus, Renè, Filipe, Paulo L., Sousa, Ana E., Ferreira, João A.
Formato: Artigo
Idioma:Inglês
Publicado em: Impact Journals LLC 2017
Assuntos:
Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5522303/
https://ncbi.nlm.nih.gov/pubmed/28465489
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.18632/oncotarget.17121
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