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Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2′-deoxyuridine
We report procedures to allow incorporation and detection of 5-ethynyl-2′-deoxyuridine (EdU) in fission yeast, a thymidine analogue which has some technical advantages over use of bromodeoxyuridine. Low concentrations of EdU (1 µM) are sufficient to allow detection of incorporation in cells expressi...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
2011
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3089489/ https://ncbi.nlm.nih.gov/pubmed/21310713 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr063 |
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