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Expression, purification, and contaminant detection for structural studies of Ralstonia metallidurance ClC protein rm1
Single-particle electron cryo-microscopy (cryo-EM) has become a popular method for high-resolution study of the structural and functional properties of proteins. However, sufficient expression and purification of membrane proteins holds many challenges. We describe methods to overcome these obstacle...
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| Publicado no: | PLoS One |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5503242/ https://ncbi.nlm.nih.gov/pubmed/28692650 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0180163 |
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