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R-loop depletion by over expressed RNase H1 in mouse B cells increases activation-induced deaminase access to the transcribed strand without altering frequency of isotype switching

R-loops, three strand structures consisting of mRNA hybridized to the complementary DNA and a single stranded DNA loop, are formed in switch regions on the heavy chain immunoglobulin locus. To determine if R-loops have a direct effect on any of the steps involved in isotype switching, we generated a...

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Detalhes bibliográficos
Publicado no:J Mol Biol
Main Authors: Maul, Robert W., Chon, Hyongi, Sakhuja, Kiran, Cerritelli, Susana M., Gugliotti, Lina A., Gearhart, Patricia J., Crouch, Robert J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5500445/
https://ncbi.nlm.nih.gov/pubmed/28065739
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2016.12.020
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