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Simple and highly efficient BAC recombineering using galK selection
Recombineering allows DNA cloned in Escherichia coli to be modified via lambda (λ) Red-mediated homologous recombination, obviating the need for restriction enzymes and DNA ligases to modify DNA. Here, we describe the construction of three new recombineering strains (SW102, SW105 and SW106) that all...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
2005
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC549575/ https://ncbi.nlm.nih.gov/pubmed/15731329 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni035 |
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