Simple and highly efficient BAC recombineering using galK selection

Recombineering allows DNA cloned in Escherichia coli to be modified via lambda (λ) Red-mediated homologous recombination, obviating the need for restriction enzymes and DNA ligases to modify DNA. Here, we describe the construction of three new recombineering strains (SW102, SW105 and SW106) that all...

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Bibliografiset tiedot
Päätekijät: Warming, Søren, Costantino, Nina, Court, Donald L., Jenkins, Nancy A., Copeland, Neal G.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Oxford University Press 2005
Aiheet:
Methods Online
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC549575/
https://ncbi.nlm.nih.gov/pubmed/15731329
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni035
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