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An improved counterselectable marker system for mycobacterial recombination using galK and 2-Deoxy-Galactose
Counterselectable markers are powerful tools in genetics because they allow selection for loss of a genetic marker rather than its presence. In mycobacteria, a widely used counterselectable marker is the gene encoding levan sucrase (sacB), which confers sensitivity to sucrose, but frequent spontaneo...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3008429/ https://ncbi.nlm.nih.gov/pubmed/20851171 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.gene.2010.09.005 |
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