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Development of a bacterial artificial chromosome (BAC) recombineering procedure using galK-untranslated region (UTR) for the mutation of diploid genes

Bacterial artificial chromosome (BAC) recombineering using galK selection allows DNA cloned in E. coli to be modified without introducing an unwanted selectable marker at the modification site. Genomes of some herpesviruses have a pair of inverted repeat sequences that makes it very difficult to int...

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Detalhes bibliográficos
Main Authors: Dai, Gan, Kim, Seongman, O’Callaghan, Dennis J., Kim, Seong K.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3388943/
https://ncbi.nlm.nih.gov/pubmed/22407056
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2012.02.010
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