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Monoclonal antibodies expression improvement in CHO cells by PiggyBac transposition regarding vectors ratios and design

Establishing stable Chinese Hamster Ovary (CHO) cells producing monoclonal antibodies (mAbs) usually pass through the random integration of vectors to the cell genome, which is sensitive to gene silencing. One approach to overcome this issue is to target a highly transcribed region in the genome. Tr...

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Vydáno v:PLoS One
Hlavní autoři: Ahmadi, Samira, Davami, Fatemeh, Davoudi, Noushin, Nematpour, Fatemeh, Ahmadi, Maryam, Ebadat, Saeedeh, Azadmanesh, Kayhan, Barkhordari, Farzaneh, Mahboudi, Fereidoun
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5491063/
https://ncbi.nlm.nih.gov/pubmed/28662065
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0179902
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