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Evaluating the efficiency of CHEF and CMV promoter with IRES and Furin/2A linker sequences for monoclonal antibody expression in CHO cells

In recent years, monoclonal antibodies (mAbs) have been developed as powerful therapeutic and diagnostic agents and Chinese hamster ovary (CHO) cells have emerged as the dominant host for the recombinant expression of these proteins. A critical step in recombinant expression is the utilization of st...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Ebadat, Saeedeh, Ahmadi, Samira, Ahmadi, Maryam, Nematpour, Fatemeh, Barkhordari, Farzaneh, Mahdian, Reza, Davami, Fatemeh, Mahboudi, Fereidoun
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5638317/
https://ncbi.nlm.nih.gov/pubmed/29023479
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0185967
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