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RNA interference rescue by bacterial artificial chromosome transgenesis in mammalian tissue culture cells

RNA interference (RNAi) is a widely used method for analysis of gene function in tissue culture cells. However, to date there has been no reliable method for testing the specificity of any particular RNAi experiment. The ideal experiment is to rescue the phenotype by expression of the target gene in...

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Détails bibliographiques
Auteurs principaux: Kittler, Ralf, Pelletier, Laurence, Ma, Chunling, Poser, Ina, Fischer, Steffi, Hyman, Anthony A., Buchholz, Frank
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 2005
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC548992/
https://ncbi.nlm.nih.gov/pubmed/15695330
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0409861102
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