A Plasmid Set for Efficient Bacterial Artificial Chromosome (BAC) Transgenesis in Zebrafish

Transgenesis of large DNA constructs is essential for gene function analysis. Recently, Tol2 transposase-mediated transgenesis has emerged as a powerful tool to insert bacterial artificial chromosome (BAC) DNA constructs into the genome of zebrafish. For efficient transgenesis, the genomic DNA piece...

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Publicat a:G3 (Bethesda)
Autors principals: Fuentes, Fernando, Reynolds, Eric, Lewellis, Stephen W., Venkiteswaran, Gayatri, Knaut, Holger
Format: Artigo
Idioma:Inglês
Publicat: Genetics Society of America 2016
Matèries:
Investigations
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4825653/
https://ncbi.nlm.nih.gov/pubmed/26818072
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.115.026344
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