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Analysis of Replicative Intermediates of Adeno-associated Virus through Hirt Extraction and Southern Blotting
Adeno-associated virus (AAV) is a small single-stranded DNA virus that requires the presence of a helper virus, such as adenovirus or herpes virus, to efficiently replicate its genome. AAV DNA is replicated by a rolling-hairpin mechanism (Ward, 2006), and during replication several DNA intermediates...
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| Udgivet i: | Bio Protoc |
|---|---|
| Main Authors: | , , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Bio-Protocol
2017
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5484404/ https://ncbi.nlm.nih.gov/pubmed/28660237 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2271 |
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