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Determination of Adeno-associated Virus Rep DNA Binding Using Fluorescence Anisotropy
Quantitative measurement of proteins binding to DNA is a requisite to fully characterize the structural determinants of complex formation necessary to understand the DNA transactions that regulate cellular processes. Here we describe a detailed protocol to measure binding affinity of the adeno-assoc...
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| Udgivet i: | Bio Protoc |
|---|---|
| Main Authors: | , , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Bio-Protocol
2017
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5466871/ https://ncbi.nlm.nih.gov/pubmed/28612042 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2194 |
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