Analysis of Replicative Intermediates of Adeno-associated Virus through Hirt Extraction and Southern Blotting

Adeno-associated virus (AAV) is a small single-stranded DNA virus that requires the presence of a helper virus, such as adenovirus or herpes virus, to efficiently replicate its genome. AAV DNA is replicated by a rolling-hairpin mechanism (Ward, 2006), and during replication several DNA intermediates...

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Bibliographic Details
Published in:Bio Protoc
Main Authors: Bardelli, Martino, Zarate-Perez, Francisco, Agundez, Leticia, Jolinon, Nelly, Linden, Michael R., Escalante, Carlos R., Henckaerts, Els
Format: Artigo
Language:Inglês
Published: Bio-Protocol 2017
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Methods Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5484404/
https://ncbi.nlm.nih.gov/pubmed/28660237
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2271
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