CRISPR/Cas9-mediated gene knockout screens and target identification via whole-genome sequencing uncover host genes required for picornavirus infection

Several groups have used genome-wide libraries of lentiviruses encoding small guide RNAs (sgRNAs) for genetic screens. In most cases, sgRNA expression cassettes are integrated into cells by using lentiviruses, and target genes are statistically estimated by the readout of sgRNA sequences after targe...

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Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Kim, Heon Seok, Lee, Kyungjin, Bae, Sangsu, Park, Jeongbin, Lee, Chong-Kyo, Kim, Meehyein, Kim, Eunji, Kim, Minju, Kim, Seokjoong, Kim, Chonsaeng, Kim, Jin-Soo
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2017
Assuntos:
Cell Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5481571/
https://ncbi.nlm.nih.gov/pubmed/28446605
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M117.782425
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