CRISPR/Cas9-mediated gene knockout screens and target identification via whole-genome sequencing uncover host genes required for picornavirus infection

Several groups have used genome-wide libraries of lentiviruses encoding small guide RNAs (sgRNAs) for genetic screens. In most cases, sgRNA expression cassettes are integrated into cells by using lentiviruses, and target genes are statistically estimated by the readout of sgRNA sequences after targe...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:J Biol Chem
Hlavní autoři: Kim, Heon Seok, Lee, Kyungjin, Bae, Sangsu, Park, Jeongbin, Lee, Chong-Kyo, Kim, Meehyein, Kim, Eunji, Kim, Minju, Kim, Seokjoong, Kim, Chonsaeng, Kim, Jin-Soo
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Biochemistry and Molecular Biology 2017
Témata:
Cell Biology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5481571/
https://ncbi.nlm.nih.gov/pubmed/28446605
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M117.782425
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!

Podobné jednotky