CRISPR/Cas9-mediated gene knockout screens and target identification via whole-genome sequencing uncover host genes required for picornavirus infection

Several groups have used genome-wide libraries of lentiviruses encoding small guide RNAs (sgRNAs) for genetic screens. In most cases, sgRNA expression cassettes are integrated into cells by using lentiviruses, and target genes are statistically estimated by the readout of sgRNA sequences after targe...

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Detalles Bibliográficos
Publicado en:J Biol Chem
Main Authors: Kim, Heon Seok, Lee, Kyungjin, Bae, Sangsu, Park, Jeongbin, Lee, Chong-Kyo, Kim, Meehyein, Kim, Eunji, Kim, Minju, Kim, Seokjoong, Kim, Chonsaeng, Kim, Jin-Soo
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Biochemistry and Molecular Biology 2017
Assuntos:
Cell Biology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5481571/
https://ncbi.nlm.nih.gov/pubmed/28446605
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M117.782425
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