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CRISPR/Cas9-mediated gene knockout screens and target identification via whole-genome sequencing uncover host genes required for picornavirus infection

Several groups have used genome-wide libraries of lentiviruses encoding small guide RNAs (sgRNAs) for genetic screens. In most cases, sgRNA expression cassettes are integrated into cells by using lentiviruses, and target genes are statistically estimated by the readout of sgRNA sequences after targe...

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Bibliografski detalji
Izdano u:J Biol Chem
Glavni autori: Kim, Heon Seok, Lee, Kyungjin, Bae, Sangsu, Park, Jeongbin, Lee, Chong-Kyo, Kim, Meehyein, Kim, Eunji, Kim, Minju, Kim, Seokjoong, Kim, Chonsaeng, Kim, Jin-Soo
Format: Artigo
Jezik:Inglês
Izdano: American Society for Biochemistry and Molecular Biology 2017
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5481571/
https://ncbi.nlm.nih.gov/pubmed/28446605
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M117.782425
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