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DNA display of folded RNA libraries enabling RNA-SELEX without reverse transcription
A method for the physical attachment of folded RNA libraries to their encoding DNA is presented as a way to circumvent the reverse transcription step during systematic evolution of RNA ligands by exponential enrichment (RNA-SELEX). A DNA library is modified with one isodC base to stall T7 polymerase...
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| Publicado no: | Chem Commun (Camb) |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5458776/ https://ncbi.nlm.nih.gov/pubmed/28220154 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c6cc09991b |
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