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High throughput sequencing analysis of RNA libraries reveals the influences of initial library and PCR methods on SELEX efficiency
The systemic evolution of ligands by exponential enrichment (SELEX) technique is a powerful and effective aptamer-selection procedure. However, modifications to the process can dramatically improve selection efficiency and aptamer performance. For example, droplet digital PCR (ddPCR) has been recent...
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| Publicado no: | Sci Rep |
|---|---|
| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5031971/ https://ncbi.nlm.nih.gov/pubmed/27652575 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep33697 |
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