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By-Product Formation in Repetitive PCR Amplification of DNA Libraries during SELEX

The selection of nucleic acid aptamers is an increasingly important approach to generate specific ligands binding to virtually any molecule of choice. However, selection-inherent amplification procedures are prone to artificial by-product formation that prohibits the enrichment of target-recognizing...

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Veröffentlicht in:PLoS One
Hauptverfasser: Tolle, Fabian, Wilke, Julian, Wengel, Jesper, Mayer, Günter
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2014
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4260880/
https://ncbi.nlm.nih.gov/pubmed/25490402
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0114693
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