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By-Product Formation in Repetitive PCR Amplification of DNA Libraries during SELEX

The selection of nucleic acid aptamers is an increasingly important approach to generate specific ligands binding to virtually any molecule of choice. However, selection-inherent amplification procedures are prone to artificial by-product formation that prohibits the enrichment of target-recognizing...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Tolle, Fabian, Wilke, Julian, Wengel, Jesper, Mayer, Günter
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4260880/
https://ncbi.nlm.nih.gov/pubmed/25490402
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0114693
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