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By-Product Formation in Repetitive PCR Amplification of DNA Libraries during SELEX

The selection of nucleic acid aptamers is an increasingly important approach to generate specific ligands binding to virtually any molecule of choice. However, selection-inherent amplification procedures are prone to artificial by-product formation that prohibits the enrichment of target-recognizing...

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Pubblicato in:PLoS One
Autori principali: Tolle, Fabian, Wilke, Julian, Wengel, Jesper, Mayer, Günter
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4260880/
https://ncbi.nlm.nih.gov/pubmed/25490402
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0114693
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