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Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications
Quantitative real-time PCR has revolutionized many aspects of genetic research, biomedical diagnostics and pathogen detection. Nevertheless, the full potential of this technology has yet to be realized, primarily due to the limitations of the threshold-based methodologies that are currently used for...
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
2004
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| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC545475/ https://ncbi.nlm.nih.gov/pubmed/15601990 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh177 |
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