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Instant evaluation of the absolute initial number of cDNA copies from a single real-time PCR curve
Amplification of a cDNA product by quantitative PCR (qPCR) is monitored by a fluorescent signal proportional to the amount of produced amplicon. The qPCR amplification curve usually displays an exponential phase followed by a non-exponential phase, ending with a plateau. Contrary to prevalent interp...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC390378/ https://ncbi.nlm.nih.gov/pubmed/15054124 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh053 |
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