Instant evaluation of the absolute initial number of cDNA copies from a single real-time PCR curve

Amplification of a cDNA product by quantitative PCR (qPCR) is monitored by a fluorescent signal proportional to the amount of produced amplicon. The qPCR amplification curve usually displays an exponential phase followed by a non-exponential phase, ending with a plateau. Contrary to prevalent interp...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Swillens, Stéphane, Goffard, Jean-Christophe, Maréchal, Yoann, de Kerchove d'Exaerde, Alban, El Housni, Hakim
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 2004
Soggetti:	NAR Methods Online
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390378/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15054124/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh053
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