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IGF1 and IGF2 specificities to the two insulin receptor isoforms are determined by insulin receptor amino acid 718

METHODS: Alanine scan of insulin receptor (IR)-B exon 11 and site-directed mutagenesis of amino acid 718 in human IR-A and IR-B were performed. Ligand affinities to wild type and mutated receptors were studied by displacement of radioactive insulin in binding assay on secreted soluble midi receptors...

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Détails bibliographiques
Publié dans:PLoS One
Auteurs principaux: Andersen, Mie, Nørgaard-Pedersen, Dorte, Brandt, Jakob, Pettersson, Ingrid, Slaaby, Rita
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2017
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5453582/
https://ncbi.nlm.nih.gov/pubmed/28570711
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0178885
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