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Physiological Mg(2+) Conditions Significantly Alter the Inhibition of HIV-1 and HIV-2 Reverse Transcriptases by Nucleoside and Non-Nucleoside Inhibitors in Vitro

Reverse transcriptases (RTs) are typically assayed in vitro with 5–10 mM Mg(2+), whereas the free Mg(2+) concentration in cells is much lower. Artificially high Mg(2+) concentrations used in vitro can misrepresent different properties of human immunodeficiency virus (HIV) RT, including fidelity, cat...

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Bibliografiske detaljer
Udgivet i:Biochemistry
Main Authors: Achuthan, Vasudevan, Singh, Kamlendra, DeStefano, Jeffrey J.
Format: Artigo
Sprog:Inglês
Udgivet: 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5453313/
https://ncbi.nlm.nih.gov/pubmed/27936595
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.6b00943
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