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Physiological Mg(2+) Conditions Significantly Alter the Inhibition of HIV-1 and HIV-2 Reverse Transcriptases by Nucleoside and Non-Nucleoside Inhibitors in Vitro
Reverse transcriptases (RTs) are typically assayed in vitro with 5–10 mM Mg(2+), whereas the free Mg(2+) concentration in cells is much lower. Artificially high Mg(2+) concentrations used in vitro can misrepresent different properties of human immunodeficiency virus (HIV) RT, including fidelity, cat...
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| Publicado no: | Biochemistry |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5453313/ https://ncbi.nlm.nih.gov/pubmed/27936595 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.6b00943 |
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