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Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA

Wild-type methyl-CpG-binding domain (MBD) proteins specifically bind symmetrically methylated DNA sequences, and assays have been developed that use these proteins for profiling DNA methylation. Here, we use directed evolution in the yeast surface display format to identify a new protein variant tha...

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Bibliografske podrobnosti
izdano v:Mol Syst Des Eng
Main Authors: Tarn, B. E., Sung, K.-J., Sikes, H. D.
Format: Artigo
Jezik:Inglês
Izdano: 2016
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5450655/
https://ncbi.nlm.nih.gov/pubmed/28580176
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/C6ME00073H
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