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Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA
Wild-type methyl-CpG-binding domain (MBD) proteins specifically bind symmetrically methylated DNA sequences, and assays have been developed that use these proteins for profiling DNA methylation. Here, we use directed evolution in the yeast surface display format to identify a new protein variant tha...
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| Publicado no: | Mol Syst Des Eng |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5450655/ https://ncbi.nlm.nih.gov/pubmed/28580176 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/C6ME00073H |
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